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. 2020 Jun 16;11:1070. doi: 10.3389/fmicb.2020.01070

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Copyright © 2020 Yan, Zhao, Xue, Zheng, Xu, Liu, Tian, Wang, He and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recruitments and/or activations of B cells from LNs and peripheral blood in mice vaccinated with rAd5-G-H. BALB/c mice (n = 10 in each group) were immunized i.m. with 10⁸ GFU rAd5-G-H, rAd5-GFP, or DMEM. Single-cell suspensions of LNs (n = 6) were collected and prepared at 3, 6, and 9 dpi, and those from peripheral blood (n = 4) were collected at 7 and 14 dpi. The cells were stained with antibodies to CD19 and CD40 and then analyzed by flow cytometry. (A,D) The gating strategies for analyzing the B cells and (B,E) representative flow cytometric plots for measuring recruited and/or activated B cells (CD19⁺ CD40⁺) are shown. Analyses of activated B cells from the LNs (C) at 3, 6, and 9 dpi and from the peripheral blood (F) at 7 and 14 dpi are presented. Statistical analyses were tested by Student’s t-test in SPSS 22.0 software. *P < 0.05, **P < 0.01, and ***P < 0.001.