FIGURE 7.
A single dose of rAd5-G-H elicited complete protection against challenge with a lethal dose of CDVQL strain in foxes. (A) Fox study design. Eight-week-old female foxes (n = 6) were immunized i.m. with 109 GFU rAd5-G-H or rAd5-GFP. Foxes injected with DMEM served as mock controls. Foxes were challenged i.m. with 100 LD50 of wild-type CDVQL strain 4 weeks after immunization. (B,C) At 0, 2, 3, and 4 weeks after immunization, blood samples were collected to detect VNAs against RABV (B) and CDV (C). (D) Survival curves of foxes challenged with CDVQL. Statistical analyses were tested by Student’s t-test in SPSS 22.0 software. *P < 0.05, **P < 0.01, and ***P < 0.001.