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. 2020 May 15;17(2):371–386. doi: 10.20892/j.issn.2095-3941.2019.0452

Figure 1.

Figure 1

KIF18B is overexpressed in osteosarcoma tissues and cells. (A) mRNA and (B) protein expression of KIF18B was higher in the three osteosarcoma cell lines than in hFOB1.19. The Mann-Whitney U test was used for statistical analysis. KIF18B mRNA expression in the osteosarcoma cell lines (HOS, U2OS, and Saos-2) was significantly higher than that in hFOB1.19 immortalized human osteoblasts, as determined via RT-qPCR using GAPDH as a reference gene (n = 3). Highest expression of KIF18B was detected in HOS cells (three times that in hFOB1.19). KIF18B protein expression in HOS, U2OS, and Saos-2 cell lines was significantly higher than that in hFOB1.19, consistent with mRNA results. (C) KIF18B is overexpressed in osteosarcoma tissues of patients. Higher KIF18B expression was detected in tumor tissues of 75% (15/20) patients. The Mann-Whitney U test was used for statistical analysis. (D) Immunohistochemical staining for KIF18B in cancer and adjacent tissues from patients with osteosarcoma.