FIG 8.

CHOP does not localize to the nucleus of C. burnetii-infected cells. Uninfected (UI), TG-treated, C. burnetii-infected, or C. burnetii-infected TG-treated THP-1 cells were processed for microscopy using an antibody against CHOP (red), and DAPI (blue) was used to visualize bacterial and host DNA. Increased CHOP was present in the nuclei of TG-treated cells (positive control) but did not localize to the nuclei of C. burnetii-infected cells or uninfected cells. CHOP nuclear translocation was decreased in TG-treated infected cells compared to that in cells with TG treatment alone, indicating C. burnetii actively prevents CHOP nuclear translocation.