FIG 2.

Susceptibility of ESCRT mutants to alkaline pH and salt stress. (A) Cells (OD₆₀₀ = 1) from 10-fold serial dilutions of the indicated strains were spotted on buffered YPD plates at the indicated pH, and plates were incubated at 30°C for 2 to 7 days. (B) Tenfold serial dilutions of cells (OD₆₀₀ = 1) of the indicated strains were spotted onto solid YPD without or with 1.5 M NaCl, 1.5 M KCl, 250 mM CaCl₂, 200 mM LiCl, or 1.5 M sorbitol, and plates were incubated at 30°C for 4 to 9 days. (C) The growth of ESCRT mutants and the WT strain was tested on yeast nitrogen base (YNB) and YNB plus bathophenanthroline disulfonate (BPS) supplemented with either hemoglobin or FeCl₃ (10 μM or 100 μM) at pH 7.0.