FIG 5.

Localization of Vps27 and laccase. (A) Expression levels of LAC1/18S rRNA after 3 h of incubation under starvation conditions. Error bars indicate SEM, n = 3 independent experiments. ****, P < 0.001 (Student’s t test). Error bars represent standard deviation. (B) C. neoformans cells expressing Lac1-GFP were incubated in YPD (Glu+) and ASN without glucose (Glu−) and observed by fluorescence (Lac1, calcofluor white) or differential interference contrast (DIC) microscopy. Bar, 5 μm. (C) Indicated cells expressing Lac1-GFP were incubated in ASN without glucose (Glu−) with FM4-64 and observed by fluorescence or differential interference contrast (DIC) microscopy. Bar, 5 μm.