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. Author manuscript; available in PMC: 2020 Jun 23.
Published in final edited form as: Clin Cancer Res. 2017 Feb 7;23(14):3769–3780. doi: 10.1158/1078-0432.CCR-16-2814

FIGURE 2. Validation of SASH1 and KRT13 as miR-9 targets and of SP1 as common target of miR-1, −133a and −150, in HNSCC cells.

FIGURE 2.

A. Graphs showing (from left to right) the normalized expression of miR-9, SASH1 and KRT13 RNA in control and miR-9 knock-down SCC9 cells. Data report the median value (±S.D.) of 3 independent experiments performed in duplicate.

B. Western blot analysis of SASH1 and KRT13 protein expressions in control and miR-9 knock-down SCC9 cells. Tubulin was used as loading control.

C. Graphs showing the normalized expression of SASH1 mRNA expressions in control and miR-9 overexpressing UMSCC1 (left) and CAL27 (right) cells. Graphs report the median value (±S.D.) of 3 independent experiments performed in duplicate.

D. Western blot analysis of SASH1 and KRT13 protein expressions in control and miR-9 overexpressing CAL27 cells. GRB2 was used as loading control.

E Western blot analysis of SP1 protein expression in UMSCC74b cells expressing miR-1, −133a and −150 alone or in combination, as indicated. Tubulin was used as loading control.

F. Graph showing the normalized expression of SP1 mRNA in UMSCC74b cells transfected as in (E). Data report the median value (±S.D.) of 3 independent experiments performed in duplicate. Statistical significance was calculated using unpaired two-tailed Student’s t-test.