FIGURE 1.

Oip5‐as1 is involved in the response to OGD/R‐induced injury. A, Oip5‐as1 expression levels in H9c2 cells and neonatal rat ventricular myocytes (NRVMs) after treatment with OGD/R. B, A schematic illustration of two sgRNA sequences and the CRISPR/dCas9‐SAM target site (grey) in the promoter region located −200 bp to −100 bp from the transcription start site (TSS) of the Oip5‐as1 gene. C, RT‐qPCR analysis of Oip5‐as1 expression in H9c2 cells transfected with the CRISPR/dCas9 SAM system. Oip5‐as1 sgRNA (2) was used in all experiments involving H9c2 cells. Cell viability (D) and LDH activity (E) of H9c2 cells transfected with Oip5‐as1 overexpressing vector (oe‐Oip5‐as1) or negative control vector (oe‐NC) under OGD/R conditions. Data are expressed as mean ± SD (n = 3). *P < .05 vs the control group. ^# P < .05 vs the OGD/R + oe‐NC group