FIGURE 6.

miR‐29a‐mediated SIRT1 downregulation inactivates the AMPK/PGC1α pathway. A, The sequences and positions of Sirt1 containing wild type and the mutant binding site of miR‐29a cloned into the pmirGLO luciferase reporter vector. B, Luciferase activity analysis in HEK293T cells co‐transfected with the reporter plasmid containing the wild‐type or mutated Sirt1 sequences and miR‐29a mimics or NC mimics. C, RT‐qPCR analysis of Oip5‐as1 and miR‐29a enrichment by the Ago2 antibody and IgG antibody in RNA immunoprecipitation experiments. D, Sirt1 expression in H9c2 cells transfected with miR‐29a mimics or inhibitors by RT‐qPCR. E, Western blot and densitometric analysis of SIRT1, p‐AMPK and PGC1α levels in H9c2 cells treated with miR‐29a inhibitors and OGD/R. Data are expressed as mean ± SD (n = 3). *P < .05 vs the mimic‐NC, inhibitor‐NC, IgG or control groups. ^# P < .05 vs the OGD/R + inhibitor‐NC group