TABLE 3.
Genotyping analysis of T0 and T1 transgenic soybean plants from five CRISPR constructs.
| T0 |
T1 |
||||||
| CRISPR Construct | Line | Bar | GFP | Cas9 | gRNA | Transgenic vs. (Total plants) | # of mutant in GmAGO7a |
| AtP5p:Cas9 (gAGO7a1/b2) | ST398-4 | + | + | + | + | 0(19) | None |
| ST398-5 | + | + | + | + | 0(27) | None | |
| AtP5p:Cas9 (gAGO7a2/b1) | ST397-1 | + | + | + | + | 11(26) | None |
| ST397-2 | + | − | − | − | 0(27) | None | |
| ST397-3 | + | − | − | − | 0(25) | None | |
| ST397-4 | + | + | + | + | 11(27) | None | |
| ST397-5 | + | + | + | + | 17(27) | None | |
| ST397-10 | + | − | − | − | T0 died | ||
| AtEC1.2e1.1p:Cas9 (gAGO7a2/b1) | ST410-4 | + | ND | + | + | 6(15) | 2 |
| ST410-7 | + | ND | + | + | 1(18) | None | |
| ST410-8 | + | ND | + | + | 5(15) | None | |
| ST410-9 | + | ND | - | - | T0 died | ||
| ST410-11 | + | ND | + | + | 0(17) | None | |
| ST410-13 | + | ND | + | + | 2(2) | None | |
| ST410-14 | + | ND | + | + | 21(23) | 7 | |
| ST410-18 | + | ND | + | + | 3(3) | None | |
| ST410-25 | + | ND | + | + | 30(36) | 4 | |
| ST410-32 | + | ND | + | + | 11(15) | None | |
| ST410-35 | + | ND | + | + | 0(14) | None | |
| ST410-36 | + | ND | + | + | 12(12) | 7 | |
| ST410-40 | + | ND | + | + | 0(17) | None | |
| ST410-44 | + | ND | + | + | 6(6) | 6 | |
| GmEC1.1p:Cas9 (gAGO7a2/b1) | ST411-6 | + | ND | − | − | 0(6) | None |
| ST411-12 | + | ND | + | + | 8(18) | None | |
| ST411-17 | + | ND | − | − | 0(9) | None | |
| ST411-18 | + | ND | + | + | 4(11) | None | |
| GmEC1.2p:Cas9 (gAGO7a2/b1) | ST412-20 | + | ND | + | + | T0 died | |
| ST412-29 | + | ND | − | − | T0 died | ||
| ST412-27 | + | ND | − | − | T0 died | ||
| ST412-31 | + | ND | − | − | 0(14) | None | |
ND, no GFP detection was performed.