TABLE 4.
Genotyping analysis of CRISPR/Cas9-induced mutations in T1 and T2 soybean plants.
| CRISPR construct | T0 Line | T1 |
T2 |
|||
| No. | Mutation (GmAGO7a) | Mutant plants (GmAGO7a) | Mutant plants (GmAGO7b) | Total plants | ||
| AtEC1.2e1.1p:Cas9 (gAGO7a2/b1) | ST410-4 | #3 | − | 0 | 0 | 21 |
| #4 | − | 0 | 0 | 18 | ||
| #5 | + | 14 (3 Homo-) | 0 | 20 | ||
| #6 | − | 0 | 0 | 19 | ||
| #14 | + | 11 (2 Homo-) | 0 | 20 | ||
| #15 | − | 0 | 0 | 15 | ||
| ST410-14 | #2 | + | 7 | 0 | 16 | |
| #3 | + | 0 | 1 | 15 | ||
| #6 | + | 9 | 0 | 15 | ||
| #19 | + | ND | ND | ND | ||
| ST410-25 | #3 | + | 7 | 0 | 16 | |
| #4 | + | 9 (2 Homo-) | 0 | 14 | ||
| #8 | + | 8 | 0 | 13 | ||
| #22 | + | ND | ND | ND | ||
| ST410-32 | #1 | − | 0 | 0 | 17 | |
| #2 | − | 0 | 0 | 20 | ||
| #5 | − | 0 | 0 | 17 | ||
| #12 | − | 0 | 0 | 18 | ||
| ST410-36 | #1 | + | 1 | 0 | 14 | |
| #2 | + | ND | ND | ND | ||
| #3 | + | 4 | 0 | 16 | ||
| #4 | − | ND | ND | ND | ||
| #6 | + | 9 | 0 | 19 | ||
| #9 | + | ND | ND | ND | ||
| #11 | + | ND | ND | ND | ||
| ST410-44 | #1 | + | 8 | 0 | 8 | |
| #2 | + | 9 | 0 | 9 | ||
| #3 | + | 10 | 0 | 10 | ||
| #4 | + | 19 | 0 | 19 | ||
| #5 | + | 20 (4 Homo-) | 0 | 20 | ||
| #6 | + | ND | ND | ND | ||
| Sum | 6 | 31 | 22 | 145 | 1 | 389 |
| GmEC1.1p:Cas9 (gAGO7a2/b1) | ST411-12 | #2 | − | 0 | 0 | 19 |
| #5 | − | 0 | 0 | 17 | ||
| #17 | − | 0 | 0 | 18 | ||
| #18 | − | 0 | 0 | 18 | ||
| ST411-18 | #6 | − | 0 | 0 | 18 | |
| #12 | − | 0 | 0 | 18 | ||
| #14 | − | 0 | 0 | 18 | ||
| #20 | − | 0 | 0 | 14 | ||
| Sum | 2 | 8 | 0 | 0 | 0 | 140 |
ND, no seeds or DNA samples were collected.