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. 2020 Jun 22;20:176. doi: 10.1186/s12866-020-01858-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Morphology of control and AgNP-treated C. albicans cells. Cell morphology of untreated and AgNP-exposed C. albicans was detected by flow cytometry after 72-h incubation in the presence of differently sized AgNPs in 75 μg/mL concentration. The percentage of aggregated, yeast-shaped and elongated cells were quantified by IDEAS 6.2 software (Amnis-EMD Millipore, Burlington, MA, USA) and the percentage of elongated cells is indicated on the figures (a). Morphology of the surface-anchored cells after 72-h incubation in the presence of AgNPs was analyzed using scanning electron microscopic images (b). In order to assess the degree of reactive oxygen species formation, mean DCF fluorescence intensity was determined on DCFDA-stained, AgNP-treated C. albicans cells. Values represent the mean ± standard deviation calculated from three independent experiments (****, P < 0.0001, unpaired t test) (c)