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PCR/sequencing primers developed in this study, their characteristics and conditions
| System name | Target gene | Primer name | Sequence (5′-3′) | Amplicon size (bp) | Tm (°C) | Specificity |
|---|---|---|---|---|---|---|
| Pan-fil cox1 PCR | cox1 | Fwd.957 | ATRGTTTATCAGTCTTTTTTTATTGG | 509 | 52.0 | Filariae |
| Rwd.1465 | GCAATYCAAATAGAAGCAAAAGT | |||||
| Wol ftsZ PCR | ftsZ | Wol.ftsZ.363.f | GGRATGGGTGGTGGYACTGG | 560 | 59.5 | Wolbachia |
| Wol.ftsZ.958.r | GCATCAACCTCAAAYARAGTCAT |
Abbreviation: Tm, melting temperature
