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. 2020 Jun 22;21:414. doi: 10.1186/s12864-020-06803-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Mapping replication origins in the T. cruzi nuclear genome. a. Graphs show the extent of DNA enrichment in S phase relative to G2, in the indicated chromosomes. For each panel, the top track displays the chromosome size. The two graphs below show positive z-scores for the ratio of read depth between early S phase and G2 samples (y-axis) in a 2.5 Kbp window across the chromosome (x-axis), for two independent replicates. Finally, the track at the bottom of each panel displays the annotated transcripts. b. Distance (in Kbp) between the closest peak of MFA-seq replicates. 129 MFA-seq peaks pair were compared: the median distance between them was 61.3 Kbp and 19 (15%) overlaped