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. 2020 Jun 22;13:42. doi: 10.1186/s12284-020-00402-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — AM-colonization of temperate rice japonica rice cultivars. Roots from AM-inoculated temperate japonica rice cultivars were collected and analyzed (10 weeks post-inoculation). a Cotton blue staining of rice roots colonized by F. mosseae (Fm) or R. irregularis (Ri). The typical fungal structures were observed, such as extraradical hyphae (eh), intracellular hyphae (ih), arbuscules (a), vesicles (v), and spores (sp). Scale bar = 100 μm. b-c Estimation of AM fungal colonization according to the Trouvelot method b F%, frequency of mycorrhiza in the root system, and c a%, arbuscule abundance in mycorrhizal parts of root fragments. d Appearance of roots of rice plants inoculated with AM fungi. Representative pictures are presented. Increased pigmentation is observed in AMF-roots (grey bar)