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. 2020 Jun 5;13:34. doi: 10.1186/s12284-020-00389-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Workflow for large-scale identification of Khib sites in rice. a Western blotting analysis of total proteins in rice seedling leaves. The gels for SDS-PAGE were stained with Coomassie Blue. b Workflow for identification of Khib modification in rice. Total proteins extracted from seedling leaves were digested to peptides by trypsin. Peptides bearing Khib sites were affinity enriched with a specific Khib antibody and subsequently analyzed by LC-MS/MS.