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Spiked stool analytical sensitivity on (a) conventional PCR, (b) nPCR and (c) qPCR using 10-fold dilutions of E. histolytica trophozoites. L, 100 bp DNA ladder; N, negative control; 1–10, 10-fold dilution of trophozoites: lane 1, 106; lane 2, 105; lane 3, 104; lane 4, 103; lane 5, 102; lane 6, 10; lane 7, 1; lane 8, 0.1; lane 9, 10− 2; lane 10, 10− 3. PCR could detect up to 1000 trophozoites while nPCR and qPCR could detect up to 100 trophozoites
