Table 1.
Reference organisms used in this study and analytical specificity evaluation results
| Strains | Serogroup or species | No. of isolates | Analytical specificity of primers | |||
|---|---|---|---|---|---|---|
| PCR | nPCR | qPCR | LAMP | |||
| Entamoeba spp. | Entamoeba histolytica HM-1:IMSS | 1 | + | + | + | + |
| Entamoeba dispar SAW760 | 1 | – | – | – | – | |
| Entamoeba moshkovskii Laredo | 1 | – | – | – | – | |
| Other enteric pathogens (n = 75) | Acinetobacter baumannii | 1 | – | – | – | – |
| Aeromonas hydrophila | 2 | – | – | – | – | |
| Bacillus subtilis | 1 | – | – | – | – | |
| Burkholderia cepacia | 1 | – | – | – | – | |
| Burkholderia pseudomallei | 1 | – | – | – | – | |
| Burkholderia thailandensis | 1 | – | – | – | – | |
| Citrobacter freundii | 1 | – | – | – | – | |
| Enterococcus faecalis | 1 | – | – | – | – | |
| Enterococcus faecium | 1 | – | – | – | – | |
| Enterococcus gallinarum | 1 | – | – | – | – | |
| Enterohemorrhagic Escherichia coli | 1 | – | – | – | – | |
| Enteroinvasive Escherichia coli | 1 | – | – | – | – | |
| Enteropathogenic Escherichia coli | 1 | – | – | – | – | |
| Enterotoxigenic Escherichia coli | 1 | – | – | – | – | |
| Escherichia coli | 1 | – | – | – | – | |
| group A Streptococcus | 1 | – | – | – | – | |
| group B Streptococcus | 1 | – | – | – | – | |
| group F Streptococcus | 1 | – | – | – | – | |
| group G Streptococcus | 1 | – | – | – | – | |
| Haemophilus influenza | 1 | – | – | – | – | |
| Klebsiella pneumoniae | 2 | – | – | – | – | |
| Leptospira biflexa ser. Patoc | 1 | – | – | – | – | |
| Leptospira interrogans ser. Canicola | 1 | – | – | – | – | |
| Leptospira interrogans ser. Hebdomadis | 1 | – | – | – | – | |
| Leptospira interrogans ser. Pomona | 1 | – | – | – | – | |
| Leptospira licerasiae ser. Varillal | 1 | – | – | – | – | |
| Listeria monocytogenes | 1 | – | – | – | – | |
| Salmonella enterica ser. Agona | 1 | – | – | – | – | |
| Salmonella enterica ser. Albany | 1 | – | – | – | – | |
| Salmonella enterica ser. Bardo | 1 | – | – | – | – | |
| Salmonella enterica ser. Bordeaux | 1 | – | – | – | – | |
| Salmonella enterica ser. Braenderup | 1 | – | – | – | – | |
| Salmonella enterica ser. Emek | 1 | – | – | – | – | |
| Salmonella enterica ser. Enteritidis | 1 | – | – | – | – | |
| Salmonella enterica ser. Hadar | 1 | – | – | – | – | |
| Salmonella enterica ser. Heidelberg | 1 | – | – | – | – | |
| Salmonella enterica ser. Java | 1 | – | – | – | – | |
| Salmonella enterica ser. Kibi | 1 | – | – | – | – | |
| Salmonella enterica ser. Kissi | 1 | – | – | – | – | |
| Salmonella enterica ser. Newport | 1 | – | – | – | – | |
| Salmonella enterica ser. Oslo | 1 | – | – | – | – | |
| Salmonella enterica ser. Paratyphi A | 2 | – | – | – | – | |
| Salmonella enterica ser. Paratyphi B | 2 | – | – | – | – | |
| Salmonella enterica ser. Paratyphi C | 1 | – | – | – | – | |
| Salmonella enterica ser. Poona | 1 | – | – | – | – | |
| Salmonella enterica ser. Regent | 1 | – | – | – | – | |
| Salmonella enterica ser. Richmond | 1 | – | – | – | – | |
| Salmonella enterica ser. Tshiongwe | 1 | – | – | – | – | |
| Salmonella enterica ser. Typhi | 2 | – | – | – | – | |
| Salmonella enterica ser. Typhimurium | 1 | – | – | – | – | |
| Salmonella enterica ser. Uppsala | 1 | – | – | – | – | |
| Salmonella enterica ser. Virchow | 1 | – | – | – | – | |
| Salmonella enterica ser. Weltevreden | 1 | – | – | – | – | |
| Shigella boydii | 2 | – | – | – | – | |
| Shigella dysenteriae | 2 | – | – | – | – | |
| Shigella flexneri | 2 | – | – | – | – | |
| Shigella sonnei | 2 | – | – | – | – | |
| Staphylococcus aureus | 1 | – | – | – | – | |
| Staphylococcus epidermidis | 1 | – | – | – | – | |
| Vibrio cincinnatiensis | 1 | – | – | – | – | |
| Vibrio fluvialis | 1 | – | – | – | – | |
| Vibrio mimicus | 1 | – | – | – | – | |
| Vibrio parahaemolyticus | 1 | – | – | – | – | |
| Vibrio vulnificus | 1 | – | – | – | – | |
| Yersinia enterocolitica | 2 | – | – | – | – | |
| Total | 78 | |||||
+, positive result; −, negative result