Fig. 4.

Silencing circDONSON sensitizes GC cells to DDP by targeting miR-802. a qRT-PCR analysis of miR-802 in AGS/DDP and HGC-27/DDP cells transfected with anti-miR-802 or anti-miR-NC. AGS/DDP and HGC-27/DDP cells were transfected with si-NC, si-circDONSON, si-circDONSON + anti-miR-NC, or si-circDONSON + anti-miR-802. b CCK-8 assay of cell proliferation capacity and IC₅₀ value of DDP in AGS/DDP and HGC-27/DDP cells after treatment with a series dose of DDP (0.125, 0.25, 0.5, 1, 2, 4, or 8 µM). c Colony formation assay of the colony-forming ability of AGS/DDP and HGC-27/DDP cells under 1 µM DDP treatment. d Apoptosis analysis of AGS/DDP and HGC-27/DDP cells under 1 µM DDP treatment using flow cytometry. e Levels detection of Cyclin D1 and p27 protein in AGS/DDP and HGC-27/DDP cells with western blot. n = 3, *P < 0.05