Fig. 5.

BMI1 is a target of miR-802 in GC cells. a Schematic of the binding sites between miR-802 and BMI1. b Dual-luciferase reporter assay in AGS/DDP and HGC-27/DDP cells co-transfected with the reporter plasmid and indicated miRNAs. c, d Levels detection of BMI1 mRNA and protein in AGS/DDP and HGC-27/DDP cells transfected with anti-miR-802 or anti-miR-NC using qRT-PCR and western blot. e–h Measurement of the mRNA and protein levels of BMI1 in normal gastric mucosa from noncancerous patients and gastric cancer tissues (N = 60), as well as in DDP responsive (N = 35) and non-responsive GC tissues (N = 25) using qRT-PCR and western blot. i, j Levels detection of the mRNA and protein of BMI1 in normal GES-1 cells, GC cell lines (AGS and HGC-27), and DDP-resistant GC cell lines (AGS/DDP and HGC-27/DDP) using qRT-PCR and western blot. k Correlation analysis between miR-802 and BMI1 expression in GC tissues. n = 3, *P < 0.05