Fig. 4.

Anti-angiogenic activity of conditioned media using in vivo Matrigel plug assay. Six-week-old nude mice were subcutaneously injected with Matrigel containing concentrated CM (c-CM). a After 7 days, mice were sacrificed, and representative Matrigel plugs were removed and photographed. Sections of each Matrigel plug were stained with H&E and Masson’s Trichrome staining. Scale bar, 200 μm. b. The number of blood vessels was counted by two independent observers and averaged. Quantitative RT-PCR analysis of c CD31 and vWF and d VEGFA, VEGFR2, and VEGFB expression levels in Matrigel plugs from implanted mice. Less neovascularization was observed in the Matrigel plug of c-CM-Spe. Data are representative of at least two independent experiments. Data are shown as mean ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001