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. 2020 Jun 22;20:582. doi: 10.1186/s12885-020-07067-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — SOP induces apoptosis and G2/M phase arrest in gastric cancer cells. a AGS cells were treated with indicated concentrations of SOP for 24 h, Annexin V-FITC/PI stain and flow cytometry analysis were performed to assess apoptosis. b Statistical analysis of the Annexin V⁺PI⁺ cell ratio in AGS cells. c Statistical analysis of the Annexin V⁺PI⁺ cell ratio in SGC7901 cells. d Western blot analysis of the expression of apoptosis related proteins in AGS cells treated with indicated concentrations of SOP for 24 h. Full-length blots/gels are presented in Supplementary Figure S4. e AGS cells were treated with indicated concentrations of SOP for 24 h, PI stain and flow cytometry analysis were performed to assess cell cycle distribution. f Statistical analysis of cell cycle phase ratio in AGS cells. g Statistical analysis of cell cycle phase ratio in SGC7901 cells treated with indicated concentrations of SOP for 24 h. h AGS cells were treated with or without 3 μM SOP for indicated hours, and then γH2AX and RAD51 expression were determined by western blot. Full-length blots/gels are presented in Supplementary Figure S4. The results are representatives of at least 3 independent experiments. Data were presented as mean ± SD. Abbreviation: SOP, Sophoridine