Figure 5.

Serotype determination from NGS reads. (A) Unique signature sequences from the capsid genes were identified for each serotype. (B) The percentage of correct serotype calls, percentage of calls returning no signature, and percentage of samples with additional calls in the serotype determination Python script are listed. (C) FASTQ files from a sample with a suspected mix-up were aligned to the PHP.eB, and AAV1 capsid sequences and the alignments were depicted. Black bars indicate a lack of consensus, whereas gray bars indicate good consensus with the reference sequence. AAV, recombinant adeno-associated virus; NGS, next-generation sequencing. Color images are available online.