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. 2020 Jun 22;16:213. doi: 10.1186/s12917-020-02431-2

Fig. 2.

Fig. 2

Analysis of ptger4 transcription in a section of normal skin (a-c) and squamous cell carcinoma (d-f) following RNAscope® mRNA in situ hybridization with hematoxylin counter stain. a & d: Native photomicrograph to be analyzed for copy number/cell, H-Score, and percentage transcript expression using HALO software with RNAscope® Modules. b & e: Target cells were manually gated (yellow line) for analysis. c & f: HALO generated probe markup from which copy number/cell, H-Score, and percentage transcript expression are calculated