Fig. 3.

Analysis of ptger4 transcription in a section of normal anal sac (a-c) and apocrine gland anal sac adenocarcinoma (d, e) following RNAscope® mRNA in situ hybridization with hematoxylin counter stain. a & d: Native photomicrograph to be analyzed for copy number/cell, H-Score, and percentage transcript expression using HALO software with RNAscope® Modules. b: Target cells were manually gated (yellow line) for analysis; no gated cells for tumor image since all displayed cells were neoplastic. c & e: HALO generated probe markup from which copy number/cell, H-Score, and percentage transcript expression are calculated