Fig. 2.

Response of cultured human DPCs to CRF. The endogenous expression of CRF and its receptors in cultured human DPCs (a) and ORSCs (b) (scale bars = 50 μm, blue: DAPI, green: IgG, CRFR1, or CRFR1/2). c, d Human DPCs were exposed to 10^− 6 M CRF for 24 h and assessed fluorescence intensity of CRFR1 and CRFR2 using immunocytochemistry (scale bars = 100 μm, blue: DAPI, green: IgG, CRFR1, red: IgG, CRFR2) and e confirmed using western blots. f, g, h The bands were quantified using ImageJ. p values compared with the normal group by t-tests, *: p < 0.05, **: p < 0.01