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. 2020 Jun 23;18:100. doi: 10.1186/s12964-020-00604-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — FBXL6 stabilizes c-MYC via HSP90AA1. a Western blot analysis of the WCL derived from SMMC-7721 cells or Hep3B cells transfected with EV or HSP90AA1 plasmids. b Western blot analysis of the WCL derived from Hep3B transfected with increased dose of Flag-FBXL6. c Western blot analysis of the WCL derived from SMMC-7721 cells transfected with EV or Flag-FBXL6ΔF-box plasmids. d The protein levels of c-Myc from FBXL6^+/+and FBXL6^−/− SMMC-7721 cells were detected by immunoblotting. e FBXL6^+/+and FBXL6^−/− SMMC-7721 cells were treated with 20 μM CHX for the indicated time. The whole cell lysate was immunoblotted with anti-c-Myc antibody. The quantification plot was based on scanning densitometry analysis using the Image J software. Relative protein levels were normalized to FBXL6^−/− control group. f The WCL from FBXL6^+/+and FBXL6^−/− SMMC-7721 cells were immunoprecipitated by Tandem Ubiquitin Binding Entity 2 (TUBE2) resin for ubiquitinated proteins enrichment and immunoblotted as indicated. g FBXL6^+/+ and FBXL6^−/− SMMC-7721 cells transfected with vector control or HSP90AA1 plasmids were subjected to western blot assay with indicated antibodies