Figure 5.

CD73 levels reduced in nonarterial calcification due to deficiency of CD73 (ACDC) calcified femoropopliteal artery exhibiting higher levels of FOXO1 (forkhead box O1 protein). Sections of femoropopliteal arteries from 20 different patients were stained with Von Kossa stain to detect calcification. Of the 20 patients, no calcification was detected in 7 vessels and the remaining 13 show calcification. Serial sections were stained with CD73, FOXO1, and TNAP antibody, and an IgG control to match concentration of TNAP at 20 µg/mL. Pixel quantification was done using ImageJ and normalized to DAPI. **P=0.0023, @@P=0.0067, $P=0.0151, $$P=0.0043, #P=0.0133. M=tunica media. I=tunica intima. L=lumen. Von Kossa scale bar represents 100 µm, fluorescent images scale bar represents 50 µm.