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. Author manuscript; available in PMC: 2020 Jun 23.
Published in final edited form as: Neurobiol Learn Mem. 2018 Nov 5;156:103–116. doi: 10.1016/j.nlm.2018.11.002

Fig. 2.

Fig. 2.

Experimental design for pre-training CNO injections using the object placement (A) and object recognition tasks (B). (C) In the object placement task, DH sham and eGFP control mice administered 2 mg/kg CNO 30 min before training spent significantly more time than chance (15 s) with the moved object 4 h after training, whereas DH-hM4Di-expressing mice administered 2 mg/kg CNO did not. (D) In the object recognition task, DH Sham, eGFP, and hM4Di mice administered 2 mg/kg CNO 30 min prior to training all spent significantly more time than chance (15 s) with the novel object during testing, suggesting intact object recognition memory 24 h after training. These findings suggest that pre-training hM4Di-mediated inactivation of the DH impairs spatial, but not object recognition, memory formation. Bars represent the mean ± SEM, *p < 0.05 relative to chance or the Control group.