Fig. 5.

NCEH1 expression is altered in a phorbol 12-myristate 13-acetate (PMA)-dependent manner during macrophage differentiation in THP1 cells. a–d THP1 cells were treated with 50 nM (shaded bars), 100 nM (closed bars), or no PMA (open bars) for 12 h. mRNA expression of RORα (a), NCEH1 (b), CD11b (c), and MMP9 (d) was analyzed by qRT-PCR, and data were normalized to 18S rRNA levels. Data are means ± standard errors (n = 4). *, p < 0.05. e THP1 cells were treated with or without 100 nM PMA for 24 h. Protein expression of RORα, NCEH1, and GAPDH was analyzed by immunoblot analysis. f Summary of RORα and NCEH1 protein levels for each treatment. Data were normalized to GAPDH protein expression. Data are means ± standard errors (n = 4). *, p < 0.05