Fig. 7.

Effects of agonist-induced RORα activation on NCEH1 expression. HepG2 cells expressing endogenous RORα were treated without (Vehicle, open bars) or with 5 μM SR1078 (closed bars) for 48 or 72 h, and RORα (a), NCEH1 (b), and BMAL1 (c) gene expression levels were evaluated. The expression levels of RORα-target genes stimulated by the RORα agonist are presented as the fold-change relative to those with the vehicle. Data are means ± standard errors (n = 3). *, p < 0.05. d THP1 cells were treated with 100 nM phorbol 12-myristate 13-acetate (PMA) for 72 h and then treated without or with 5 μM SR1078 for 24 h. Protein expression of NCEH1 and GAPDH was analyzed by immunoblotting. e Summary of NCEH1 protein levels with each treatment. Data were normalized to GAPDH protein expression. Data are means ± standard errors (n = 4). *, p < 0.05