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. Author manuscript; available in PMC: 2020 Jun 23.
Published in final edited form as: FEBS J. 2016 Jun 6;283(13):2476–2493. doi: 10.1111/febs.13758

FIGURE 2. Internalization of SorLA.

FIGURE 2.

A) The surface of polarized MDCK cells was labelled with SorLA antibody, and internalization was followed for various periods of time. B) Cells, treated as in A, were also stained for the cellular marker EEA1 (for sorting endosomes). The enlargements and arrows indicates areas of co-localization. C) Cells transfected with SorLA and mCherry-TfR were immunostained against SorLA and mCherry, as a marker of CRE. Arrows indicate areas of colocalization. D) Endocytosis of 20C11 anti-SorLA in neurons after 5–60 minutes. Arrows indicate areas of colocalization with EEA1 in the enlargements shown. Scalebars: 10 μm for MDCK cells and 50 μm for neurons.