Fig. 2.

Time dependence of PLT- and TGF-β-induced expression of EMT-related genes in TE11A cells. TE11A cells at confluence in 24-well plates were treated with TGF-β (20 ng/mL) or platelets (~ 2 × 10⁷/mL), EGTA (2 mM), or platelets plus EGTA for 5 h or 20 h, and RNA was extracted. Expression of the EMT-related genes, vimentin (a), N-cadherin (b), E-cadherin (c), claudin-1 (d), PDPN (e), and PAI-1 (f) was analyzed by real-time PCR. Values are mean ± range of duplicate culture wells from one representative experiment. Note the presence of EGTA (2 mM) potentiated gene alterations by platelets as compared with platelets alone, the presence of ETGA alone had no effect on basal expression levels of those genes