Fig. 5.
Platelet aggregation-inducing activity of PDPN knockout TE11A cells. In each set of assays (a–c), the same number of parental TE11A, clone 2, or clone 4 cells, or PBS (vehicle) was added to platelet-rich plasma samples in cuvettes on a 4-channel aggregometer. Aggregation was monitored by the change in turbidity. Traces shown are one representative result obtained at the final cell density of 5 × 105/mL (a), 4 × 105/mL (b), or 3.3 × 105/mL (c). In d, the odds for successful full aggregation (transparency > 60% as in a), induced by the threshold number of cells (3.3–10 × 105/mL) is summarized from a total of 10 assay sets from three independent experiments. There were no significant differences