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. 2020 Jun 23;20:263. doi: 10.1186/s12935-020-01328-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Effect of PDPN knockout on PLT- or TGF-β-induced expression of EMT-related genes in TE11A cells. Parental, clone 2, or clone 4 TE11A cells at confluence in 24-well plates were treated with TGF-β (20 ng/mL) or platelets (~ 2 × 10⁷/mL) plus EGTA (2 mM) for 18 h. The RNA was then extracted, and the expression of the EMT-related genes, vimentin (a), N-cadherin (b), E-cadherin (c), claudin-1 (d), PDPN (e), and PAI-1 (f), was analyzed by real-time PCR. In each left side graph, relative level of expression is shown with respect to non-treated parental TE11A clone, whereas in the right side graph, fold induction is expressed with respect to the basal level of each clonal cells. The inset graph in (a, b, f) shows the magnified bars for the basal level of expression of each cell clone. Values represent the mean ± SEM of quadruplicate culture wells from one representative experiment. *P < 0.001 and ***P < 0.001 vs. non-treated parental cells by one-way ANOVA and Tukey’s test, ^###P < 0.001 vs. parental cells of respective treatment group by two-way ANOVA and Bonferroni’s test

Fig. 6 — Effect of PDPN knockout on PLT- or TGF-β-induced expression of EMT-related genes in TE11A cells. Parental, clone 2, or clone 4 TE11A cells at confluence in 24-well plates were treated with TGF-β (20 ng/mL) or platelets (~ 2 × 10⁷/mL) plus EGTA (2 mM) for 18 h. The RNA was then extracted, and the expression of the EMT-related genes, vimentin (a), N-cadherin (b), E-cadherin (c), claudin-1 (d), PDPN (e), and PAI-1 (f), was analyzed by real-time PCR. In each left side graph, relative level of expression is shown with respect to non-treated parental TE11A clone, whereas in the right side graph, fold induction is expressed with respect to the basal level of each clonal cells. The inset graph in (a, b, f) shows the magnified bars for the basal level of expression of each cell clone. Values represent the mean ± SEM of quadruplicate culture wells from one representative experiment. *P < 0.001 and ***P < 0.001 vs. non-treated parental cells by one-way ANOVA and Tukey’s test, ^###P < 0.001 vs. parental cells of respective treatment group by two-way ANOVA and Bonferroni’s test