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. 2020 Jun 23;22:69. doi: 10.1186/s13058-020-01303-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Examples of immunohistochemical staining results. a, d, g Hematoxylin and eosin images of the tissue microarray cores. b, e, h Images of PD-L1 staining. c, f, i Magnified images of the boxed areas in b, e, and h. Arrow, tumor-infiltrating immune cells. Arrowhead, tumor cells. The first row (a–c) represents a case with positive staining in the tumor cells and negative staining in the immune cells. The second row (d–f) represents a case with positive staining in the immune cells and negative staining in the tumor cells. The third row (g–i) represents a case with positive staining in both the immune cells and the tumor cells. Original magnification: a, b, d, e, g, h × 40; c, f, i × 100