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. 2020 Jun 23;18:99. doi: 10.1186/s12964-020-00591-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Phosphorylation of HH pathway components after 15 min treatment. a SAG impairs GLI phosphorylation after 15 min stimulation. b Vismodegib binding induces delocalization of Smoothened in the primary cilium. c Heatmap of regulated and identified phosphosites after 15 min SAG and vismodegib treatment. Blue and red ellipses, phosphosites down- or upregulated compared to control. All quantified phosphopeptides of phosphoproteins after 15 min treatment assigned to sonic hedgehog signaling search were considered for the heatmap without applying any cutoff value. SAG/DMSO represents the ratio determined by the mean of the proteome-normalized intensities detected after SAG treatment (N = 3) divided by the mean of proteome-normalized intensities after DMSO (N = 3). Vismo/DMSO represents the ratio determined by the mean of proteome-normalized intensities detected after vismodegib treatment (N = 3) divided by the mean of proteome-normalized intensities after DMSO (N = 3). Significantly regulated phosphopeptides between SAG and vismodegib treatment are highlighted in bold