Table 2.
Antibodies used for flow cytometric analysis of T cells.
| Marker | Fluorochrome | Clone | Manufacturer | Catalog # | Staining |
|---|---|---|---|---|---|
| CD3 | Alexa Fluor 488 | SP34–2 | Becton Dickinson |
557705 | Surface |
| IL-2 | PerCP-Cy5.5 | MQ1-17H12 | Becton Dickinson |
560708 | Intracellular |
| TNFα | Alexa Fluor 700 | MAB11 | Becton Dickinson |
557996 | Intracellular |
| Live-Dead | Aqua Dead Cell Stain Kit |
ThermoFisher | L34965 | Live-dead | |
| CD8 | Brilliant Violet 605 | SK1 | Becton Dickinson |
564115 | Surface |
| CD4 | Brilliant Violet 650 | L200 | Becton Dickinson |
563737 | Surface |
| CD69 | PE | FN50 | Becton Dickinson |
560968 | Intracellular |
| IFN𝛾 | PE-Cy7 | B27 | Becton Dickinson |
557643 | Intracellular |