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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: J Neurochem. 2020 Mar 2;153(2):216–229. doi: 10.1111/jnc.14981

Figure 1. Flow chart of experimental procedure and groups for animal experiments.

Figure 1.

The experimenter decided the region that would be studied and the sex of the animal was recorded. If the animal was female, stage of estrous was determined by vaginal swab after the animal had been anesthetized. Surgery was performed under urethane anesthesia and the electrodes implanted. Spontaneous adenosine was measured for 4 hr. under anesthesia in the selected brain region. The animal was sacrificed by decapitation and a post experiment calibration of the electrode performed. The data were analyzed by software to find the adenosine transients and then statistical analysis performed.