Skip to main content
[Preprint]. 2020 Jun 12:2020.06.11.147199. [Version 1] doi: 10.1101/2020.06.11.147199

Figure 2: Temperature dependence and specificity of phase separation.

Figure 2:

A) N-protein (green) phase separates in a temperature-dependent manner (upper panel). Temperature-dependence is shifted when viral 5’-End RNA (magenta) is present (lower panel). B) Quantification of average protein intensity from (B) based on fluorescence intensity. C) Quantification of droplet area from (B). D) Quantification of protein/RNA ratio based on fluorescence intensity. E) 5’-End (yellow, upper panel) is recruited into preformed 5’-End /N-protein droplets (pink and green) but Nucleocapsid RNA (yellow, lower panel) is not efficiently recruited. F) Quantification of (E) showing intensity of second RNA added to preformed droplets. G) Mixing 5’-End and Frameshifting-region RNAs makes N-protein condensates with intermediate properties. Scale bar, 8 μm unless otherwise noted. Violin plots are scaled to have equal widths. Outliers not shown.