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. 2020 Jun 23;15(6):e0234958. doi: 10.1371/journal.pone.0234958

Fig 8. SDS-PAGE, western blot and zymogram analysis of recombinant protease 1147.

Fig 8

(A) SDS-PAGE analysis of recombinant protease 1147. Lane 1 and 4) crude extract of recombinant BL21, lane2) crude extract of non-recombinant BL21 (negative control), lane 3) Ni-NTA purified, and lane 5) heat shock purified recombinant protease 1147. (B) Native-PAGE zymogram analysis of 6) Ni-NTA affinity column and, 7) heat shock single-step method purified protease 1147, 8) crude extract of non-recombinant BL21 (negative control). (C) Western blot analysis of (9) Ni-NTA affinity column purified protease 1147, and (10) negative control.