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. 2020 Jun 23;15(6):e0234958. doi: 10.1371/journal.pone.0234958

Fig 10. Intrinsic and extrinsic fluorescence spectroscopy of the protease 1147.

Fig 10

(A) Intrinsic fluorescence spectroscopy of protease 1147 in the temperature range of 40–100°C exhibited a decrease in fluorescence intensity in temperature higher than 80°C and a blue shift in 100°C. (B) Intrinsic fluorescence spectroscopy of protease 1147 in the pH range of 1–12 showed fluorescence decline in pH higher than 9 and lower than 3. A blue and red shift was observed in acidic and basic pH, respectively. (C) Intrinsic fluorescence spectroscopy of protease 1147 in the presence of 1% Tween 20 and SDS, indicated a slight reduction of fluorescence intensity for SDS and a huge increase and red shift for Tween 20. (D) Extrinsic fluorescence spectroscopy of protease 1147 in the temperature range of 40–100°C exhibited a slight increase in fluorescence strength for temperature below 80°C and a significant increase for 90 and 100°C. (E) Extrinsic fluorescence spectroscopy of protease 1147 in the pH range of 1–12 showed huge fluctuation and blue shift of fluorescence strength in strongly acidic (pH 1–3) solution. (F) Extrinsic fluorescence spectroscopy of protease 1147 in the presence of 1% Tween 20 and SDS indicated a slight increase of fluorescence intensity for SDS and a huge increase for Tween 20.