Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Step | Volume (ml) | Total Activity (U) | Total Protein (mg) | Specific Activity (U/mg protein) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|---|
| Crude Extract | 50 | 14.1 | 49.2 | 0.28 | 100 | 1 |
| Ni-NTA | 4 | 12.4 | 0.7 | 17.7 | 87.9 | 62.3 |
| Thermal shock | 4 | 10.3 | 0.8 | 12.9 | 73.1 | 45.4 |
