Table 3. Thermodynamic parameters for irreversible thermal inactivation of protease 1147.
Parameters | kin (m-1) | t1/2 (min) | D value (min/m-1) | Ea# (kJ/mol) | ΔH# (kJ/mol) | ΔG# (kJ/mol) | ΔS# (JmolK-1) | Ref. |
---|---|---|---|---|---|---|---|---|
Temperature | This study | |||||||
60°C | 2.10 × 10−3 | 330.07 | 1318.52 | 50.31 | 47.54 | 98.71 | 154.70 | |
70°C | 8.70 × 10−3 | 79.67 | 327.82 | 47.54 | 97.83 | 147.60 | ||
80°C | 10.40 × 10−3 | 66.65 | 282.23 | 47.37 | 100.27 | 149.85 | ||
90°C | 17.10 × 10−3 | 40.53 | 176.51 | 47.28 | 101.56 | 149.51 | ||
100°C | 31.20 × 10−3 | 22.27 | 99.40 | 47.20 | 102.52 | 148.29 | ||
Other thermostable proteases | ||||||||
60°C | 39 × 10−3 | 7.95 (h) | 59.05 | 105.50 | 140.23 | 90.84 | 148.31 | [25] |
60°C | 12.40 × 10−3 | 45 | - | 34.34 | 31.12 | 93.17 | -184.75 | [26] |
60°C | 4.41 × 10−3 | 82.42 | 4.56 (h) | 20.96 | 6.36 | 92.23 | -0.26 | [27] |
60°C | 27.51 × 10−3 | 25.19 | - | 91.80 | 111.26 | 113.92 | 57.03 | [28] |
60°C | 15.83 × 10−3 | 19.62 | 155.6 | 187.4 | 184.63 | 68.15 | 349.79 | [29] |
60°C | 19.50 × 10−3 | 35.54 | - | 93.81 | 91.14 | 92.76 | -8.39 | [30] |
60°C | 35 × 10−3 | 19.80 | 65.80 | 37.19 | 34.42 | 102.53 | -204.43 | [31] |
60°C | 50 × 10−3 | 34 | - | 69 | 66.98 | 90.2 | -69.5 | [32] |