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. 2020 Jun 23;15(6):e0234958. doi: 10.1371/journal.pone.0234958

Table 3. Thermodynamic parameters for irreversible thermal inactivation of protease 1147.

The parameters were measured after 120 min incubation at various temperatures, and the values obtained at 60°C compared with other studied thermostable proteases.

Parameters kin (m-1) t1/2 (min) D value (min/m-1) Ea# (kJ/mol) ΔH# (kJ/mol) ΔG# (kJ/mol) ΔS# (JmolK-1) Ref.
Temperature This study
60°C 2.10 × 10−3 330.07 1318.52 50.31 47.54 98.71 154.70
70°C 8.70 × 10−3 79.67 327.82 47.54 97.83 147.60
80°C 10.40 × 10−3 66.65 282.23 47.37 100.27 149.85
90°C 17.10 × 10−3 40.53 176.51 47.28 101.56 149.51
100°C 31.20 × 10−3 22.27 99.40 47.20 102.52 148.29
Other thermostable proteases
60°C 39 × 10−3 7.95 (h) 59.05 105.50 140.23 90.84 148.31 [25]
60°C 12.40 × 10−3 45 - 34.34 31.12 93.17 -184.75 [26]
60°C 4.41 × 10−3 82.42 4.56 (h) 20.96 6.36 92.23 -0.26 [27]
60°C 27.51 × 10−3 25.19 - 91.80 111.26 113.92 57.03 [28]
60°C 15.83 × 10−3 19.62 155.6 187.4 184.63 68.15 349.79 [29]
60°C 19.50 × 10−3 35.54 - 93.81 91.14 92.76 -8.39 [30]
60°C 35 × 10−3 19.80 65.80 37.19 34.42 102.53 -204.43 [31]
60°C 50 × 10−3 34 - 69 66.98 90.2 -69.5 [32]