Table 5. Effects of different temperatures, pH values, SDS, and Tween 20 on the protease 1147 secondary structure and protease activity.

Protease 1147	α-helix (%)	β-sheets (%)	β-turn (%)	Random (%)	Activity (%)
50°C	16.9	31.4	20.5	48.6	90
60°C	17.0	31.8	20.5	48.5	100
70°C	16.7	31.4	20.7	48.1	80
80°C	16.5	31.0	20.9	47.9	60
90°C	16.5	30.9	20.9	47.7	50
100°C	17.2	29.8	20.9	48.3	5
pH 2	16.2	31.0	20.9	48.9	NA
pH 3	16.0	31.4	20.4	50.2	50
pH 4	16.4	31.6	20.8	48.3	70
pH 6	16.3	32.3	20.9	48.5	90
pH 7	16.5	32.5	20.7	49.3	100
pH 8	16.7	32.1	20.7	48.3	80
pH 10	16.2	31.8	20.8	49.7	20
pH 11	16.5	31.2	20.7	49.3	NA
pH 12	20.0	29.1	21.8	57.4	NA
SDS	16.4	32.5	20.8	48.6	100
Tween 20	17.0	31.7	20.8	48.6	300