Fig 7. Attenuated trabecular bone loss phenotype in Lmna^HSA-cko mice by IL-6 KO.

(A) Representative μCT 3D images of femurs from 3-month-old Lmna^f/f, Lmna^HSA-cko, IL-6–KO, and IL-6–KO; Lmna^HSA-cko littermates. (B-F) Quantification analyses by direct model of μCT analysis. Three different male mice of each genotype per group were examined blindly. *P < 0.05, **P < 0.01, significant difference. (G) The serum osteocalcin levels were measured by ELISAs. The values shown are means ± SD from three males per genotype. (H) ELISA analysis of serum PYD levels. The values presented are means ± SD (n = 3). *P < 0.05, **P < 0.01, significant difference. The underlying data for this figure can be found in S1 Data. μCT, microcomputer tomographic; BV/TV, bone volume over total volume; CB, cortical bone; cko, conditional knockout; HSA, human alpha-skeletal actin; IL, interleukin; KO, knockout; Lmna, lamin A/C gene; Lmna^f/f, floxed Lmna mice; Lmna^HSA-cko, skeletal muscle–specific Lmna-cko mice; PYD, pyridinoline; Tb.N, trabecular bone number; Tb.Sp, trabecular bone space; Tb.Th, trabecular bone thickness.