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. 2020 Jun 9;18(6):e3000728. doi: 10.1371/journal.pbio.3000728

Fig 4. Analysis of BPS composition and structure.

Fig 4

(A) 1H NMR spectra of concentrated supernatants from BPS+ ΔwzaX ΩpilA and BPS− ΔwzaB ΩpilA cultures. (B) 1H–13C HSQC spectrum of deacetylated acidic polysaccharide originally isolated from ΔwzaX ΩpilA supernatant. Analysis was performed at 27°C, 500 MHz. Resonance peak colors: black, C–H; green, C–H2. (C) Negative-mode high cone voltage (180 V) ESI-MS of deacetylated acidic polysaccharide from ΔwzaX ΩpilA supernatant. (D) Chemical structure of the BPS polymer tetrasaccharide RU. BPS, biosurfactant polysaccharide; d-ManNAc, N-acetyl-d-mannosamine; d-ManNAcA, N-acetyl-d-mannosaminuronic acid; ESI-MS, electrospray ionization mass spectrometry; HSQC, heteronuclear single quantum correlation; RU, repeating unit.