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. Author manuscript; available in PMC: 2021 Feb 15.
Published in final edited form as: J Neurosci Methods. 2019 Dec 4;332:108533. doi: 10.1016/j.jneumeth.2019.108533

Figure 1. Comparison of iPSC and ESC derivation and expansion for downstream differentiation assays.

Figure 1.

Human ESCs are typically derived from cleavage-stage embryos cultured to the blastocyst stage, followed by inner cell mass mechanoisolation, expansion in vitro, and clonal isolation/expansion of ESC lines. Conversely, iPSC derivation typically begins with the somatic cell of choice being transduced with a reprogramming cocktail to initiate reprogramming. Reprogrammed cells are then allowed to proliferate and expand, prior to clonal expansion and derivation of individual clonal lines. These images were created using BioRender.