Table 62-2.

Advantages and Limitations of Gene-based Vectors for Vaccines

Advantages	Limitations
Potent immunogenicity in animal and human infectious diseases	High level immunity to some vectors in humans
Ability to induce cellular immunity ± humoral immunity	Need for qualified packaging cell lines
Relative ease of production for many viral and non-viral vectors	Induction of anti-vector immunity following initial injection of viral vaccines
Ease of analysis and screening in the laboratory	Potential complexity with multiple vectors in prime-boost
Favorable safety profile and lack of persistence in vivo	Limited long-term safety data
Efficient transduction of cells and reasonable production capability	Need to develop large scale manufacturing processes
Many potential prime-boost combinations