Figure 5:

a) DNA is captured by the sensing pore and pulled into the cavity in between the two membranes. b) In the “driven trapping” mode, other DNA molecules can continue to be captured into the cavity though the sensing pore and can also escape through the nanofilter under the influence an applied voltage. c) The voltage is reversed and any DNA trapped in the cavity is recaptured by the sensing pore. Renders are for conceptual illustration only; geometry and DNA are not to scale. d) A representative current trace showing the transitional region of a driven trapping experiment for an applied voltage of ±200 mV for device D. e) Recapture probability for increasing loading durations in the driven trapping configuration. 7 kbp dsDNA was used for all driven trapping experiments. Three nanofiltered nanopores (circles) and two standard control nanopores (inverted triangles) were used. In alphabetical order, sensing pores had diameters of 10.0 nm (736 loading events), 7.8 nm (90 loading events), 8.1 nm (410 loading events), 7.9 nm (790 loading events), and 8.6 nm (577 loading events). Error bars are estimated using simple Poisson statistics.